3D microphysiological systems (MPS) are making a mark on drug and chemical safety testing.
Human 3D models are more relevant to human safety than in vivo models— as well as being faster and cheaper to test, while contributing to the 3Rs goal to replace, reduce and refine animal model use.
But how do we fully access the biology underlying 3D experiments and the molecular information contained within them, beyond the parameters of growth and viability?
This is how SpheroMatrices overcomes the following challenges associated with 3D models:
|3D CHALLENGE||SPHEROMATRICES SOLUTION|
|Poor antibody penetration||Microtissues are formalin-fixed, allowing for use of heat-induced epitope retrieval (HIER) and multiplexing protocols.|
|Lack of automated staining methodologies||SpheroMatrices integrates with slide-based automated image analysis/histology robotics technologies.|
|Imaging-light scatter and fluorescence quenching||With Spheromatrices technology, we image physical serial sections of the microtissue thus overcoming issues with light scatter and quenching seen with whole mount preparations.|
|Large raw data file size, demanding special software and processing||More easily processed representative data is obtained by imaging 6µm sections of replicate tissues.|
|High cost, low efficiency of 3D microtissue experiments||With serial sections, it is possible to perform multiple analyses from one experiment, and as microTMA manufacture is performed subsequent to live cell biochemical assays, maximum value is derived from a single plate.|
|Histopathological characterization of model||MicroTMA sections can be H&E stained to verify histology and visualize cell types.|