3D cell cultures are transforming drug and chemical safety testing.
Human organotypic models are more relevant to human safety than animal models— as well as being faster, cheaper, and reducing animal use.
But how do we fully access the biology behind our 3D experiments and assess molecular information, beyond the parameters of growth and viability?
This is how SpheroMatrices overcomes commonly perceived challenges associated with 3D cultures:
|3D CHALLENGE||SPHEROMATRICES SOLUTION|
|Poor antibody penetration||Spheroid tissues are formalin-fixed, allowing for application of heat-induced epitope retrieval (HIER) and multiplexing protocols.|
|Lack of high throughput staining methodologies||SpheroMatrices integrates with established automated image analysis/histology robotics technologies.|
|Imaging-light scatter and fluorescence quenching||Spheroid tissue microarray (TMA) technology makes serial sections through the spheroid and thus overcomes issues with light scatter and quenching associated with whole mount preparations.|
|Large raw data file size, demanding special software and processing||More easily processed representative data is obtained by collecting 6µm sections of replicate tissues.|
|High cost, low efficiency of 3D model experiments||TMA manufacture is performed subsequent to live cell biochemical assays; serial sections enable multiplex analyses from a single experiment, maximizing the ouput from costly 3D experiments.|
|Histopathological characterization of model||Parallel TMA sections can be H&E stained to verify histological structure and visualize cell types.|